The results obtained indicate that after 60 days of incubation Pseudomonas aeruginosa I.1.1.6 showed higher TPH biodegradation efficiency (64%) than that of Brevibacterium casei I.2.1.7 (58%). TPH were quantitatively monitored, gas chromatographic (GC) and high performance liquid chromatographic (HPLC) analysis were preformed to follow up the biodegradation process. The microcosms were inoculated at 30☌ for 60 days with a water content of 60%wt. Two promising isolates, Pseudomonas aeruginosa I.1.1.6 and Brevibacterium casei I.2.1.7, previously isolated from Egyptian hydrocarbon contaminated soil, were used. In this work, biodegradation experiments in artificially oil contaminated soil microcosms (30 cm × 26 cm × 8 cm) of shallow polypropylene plastic trays were carried out with special emphasis on biodegradation of polycyclic aromatic hydrocarbons (PAHs). In Egypt, a large number of contaminated sites exist as a result of different crude oil activities. Petroleum refining, transportation and storage are sources of soil contamination.
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